Ionic dissolution products of NovaBone promote osteoblastic proliferation via influences on the cell cycle

This study investigated the effects of the ionic dissolution products of NovaBone on osteoblastic proliferation and cell cycle regulation. MG63 osteoblast-like cells were cultured in NovaBone-conditioned Dulbecco's Modified Eagle's Medium (DMEM) or control DMEM for 10 days. The concentration of silicon ions was significantly higher in NovaBone-conditioned DMEM than control DMEM. MG63 cells cultured in NovaBone -conditioned DMEM exhibited greater proliferation on days 1 and 4 than control cells. There were increased proportions of Novabone-conditioned DMEM-cultured cells in the S and G(2)/M phases, and decreased proportions in the G(0)/G(1) phase on days 1 and 4 versus control cells, while no differences were observed on days 7 and 10 between the two groups. Bone morphogenic protein 2 production increased in both groups, but was significantly higher for the NovaBone-conditioned DMEM-cultured cells on day 10 compared with the controls. In conclusion, the NovaBone ionic dissolution products, particularly the silicon ions, promoted proliferation of MG63 osteoblast-like cells in vitro via influences on the cell cycle.