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Updated: Feb 11, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
FANCI binds branched DNA and is monoubiquitinated by UBE2T-FANCL
Simonne Longerich1, Joseph San Filippo, Dongqing Liu
1Department of Molecular Biophysics, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Abstract:
FANCI is integral to the Fanconi anemia (FA) pathway of DNA damage repair. Upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 and relocalizes to chromatin, where it functions with monoubiquitinated FANCD2 to facilitate DNA repair. We show that FANCI and its C-terminal fragment possess a DNA binding activity that prefers branched structures. We also demonstrate that FANCI can be ubiquitinated on Lys-523 by the UBE2T-FANCL pair in vitro. These findings should facilitate future efforts directed at elucidating molecular aspects of the FA pathway.
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