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Protease-resistant core form of Bacillus thuringiensis Cry1Ie: monomeric and oligomeric forms in solution
Shuyuan Guo1, Yancai Zhang, Fuping Song
1School of Life Science and Technology, Beijing Institute of Technology, 100081, Beijing, China.
Abstract:
Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC(50)) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 microg/ml, respectively.
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