Related Experiment Video
Updated: Jun 21, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
Multi-wavelength HPLC fingerprints from complex substances: An exploratory chemometrics study of the Cassia seed
Yongnian Ni1, Yanhua Lai, Sarina Brandes
1Stake Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China. ynni@ncu.edu.cn
Abstract:
Multi-wavelength fingerprints of Cassia seed, a traditional Chinese medicine (TCM), were collected by high-performance liquid chromatography (HPLC) at two wavelengths with the use of diode array detection. The two data sets of chromatograms were combined by the data fusion-based method. This data set of fingerprints was compared separately with the two data sets collected at each of the two wavelengths. It was demonstrated with the use of principal component analysis (PCA), that multi-wavelength fingerprints provided a much improved representation of the differences in the samples. Thereafter, the multi-wavelength fingerprint data set was submitted for classification to a suite of chemometrics methods viz. fuzzy clustering (FC), SIMCA and the rank ordering MCDM PROMETHEE and GAIA. Each method highlighted different properties of the data matrix according to the fingerprints from different types of Cassia seeds. In general, the PROMETHEE and GAIA MCDM methods provided the most comprehensive information for matching and discrimination of the fingerprints, and appeared to be best suited for quality assurance purposes for these and similar types of sample.
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Resolution Mass Spectrometry (HRMS)
High-Performance Liquid Chromatography: Types of Detectors
High-Performance Liquid Chromatography: Elution Process
