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Updated: Feb 10, 2026

Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing
Published on: January 12, 2024
Thermophilic biohydrogen production by an anaerobic heat treated-hot spring culture
Dogan Karadag1, Annukka E Mäkinen, Elena Efimova
1Tampere University of Technology, Department of Chemistry and Bioengineering, Tampere, Finland. dkaradag@yildiz.edu.tr
Abstract:
Batch experiments were conducted to investigate the thermophilic biohydrogen production using an enrichment culture from a Turkish hot spring. Following the enrichment, the culture was heat treated at 100 degrees C for 10 min to select for spore-forming bacteria. H(2) production was accompanied by production of acetate, butyrate, lactate and ethanol. H(2) production was associated by acetate-butyrate type fermentation while accumulation of lactate and ethanol negatively affected the H(2) yield. H(2) production was highest in the temperature range from 49.6 to 54.8 degrees C and optimum values for initial pH and concentrations of iron, yeast extract and glucose were 6.5, 40 mg/l, 4-13.5 g/l, respectively. PCR-DGGE profiling showed that the heat treated culture consisted of species closely affiliated to genus Thermoanaerobacterium.
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