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Updated: Jun 21, 2026
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
Published on: August 13, 2014
[Cytocompatibility study of Arg-Gly-Asp-recombinant spider silk protein/poly vinyl alcohol scaffold]
Hongxin Wang1, Meihong Wei, Zhengxiang Xue
1College of Life Sciences of Fujian Normal University, Fuzhou Fujian, 350108, PR China.
Objective:
To evaluate the cytocompatibility of Arg-Gly-Asp-recombinant spider silk protein (pNSR16)/ poly vinyl alcohol (PVA) through in vitro cytotoxicity experiment and cell-material co-culture experiment.
Methods:
pNSR16/PVA scaffold and its extraction were prepared by using solvent casting/particulate leaching method, and NIH-3T3 cells were cultivated with the extraction in vitro. The cytotoxicity of scaffold was analyzed using MTT assay 1, 3 and 5 days after culture. Scanning electron microscope and HE staining observation were conducted 2, 4 and 6 days after culturing NIH-3T3 cells on the pNSR16/PVA scaffold. Immunohistochemistry detection was performed 6 days after co-culture. Adhesion, growth and expression of the cells on the scaffold were observed.
Results:
The cytotoxicity of pNSR16/PVA scaffold was in grade 0. Scanning electron microscope observation: the cells covered the surface of the scaffold and were arranged in a directional manner 4 days after co-culture. HE staining: the cells adhered to and grew on the surface of scaffold, and migrated into the scaffold with the increase of culture duration. Immunohistochemistry detection: bFGF was secreted by NIH-3T3 cells, and the cells differentiated normally.
Conclusion:
pNSR16/PVA scaffold has a satisfactory cytocompatibility and may be an ideal tissue engineered scaffold material.
