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A device for automated hydrodynamic shearing of genomic DNA
1Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
Biotechniques
|July 15, 2009
Abstract:
We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000-12,000 bp by using various fluid flow rates and screens with 0.5-10-microm pores. The utilization of disposable screens eliminates sample cross-contamination and the tendency of device clogging, commonly encountered in single-orifice shearing devices.
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