Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expanded transition state analogues.

G M Blackburn1, G Kingsbury, S Jayaweera

  • 1Department of Chemistry, Krebs Institute, Sheffield University, UK.

Ciba Foundation Symposium
|January 1, 1991
PubMed
Summary

Researchers are designing better transition state analogues to create more efficient catalytic antibodies. New methods like atom substitution and insertion aim to improve antibody catalysis for various chemical reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1.

Communications biology·2022
Same author

Antibodies from Rabbits Immunized with HIV-1 Clade B SOSIP Trimers Can Neutralize Multiple Clade B Viruses by Destabilizing the Envelope Glycoprotein.

Journal of virology·2021
Same author

Evolution of catalytic centers of antibodies by virtual screening of broad repertoire of mutants using supercomputer.

Doklady. Biochemistry and biophysics·2017
Same author

Is IgM-like dislocation a common feature of antibody function?

Immunology today·2014
Same author

Reflections on biocatalysis involving phosphorus.

Biochemistry. Biokhimiia·2012
Same author

Evaluation of a nonlinear Hertzian-based model reveals prostate cancer cells respond differently to force than normal prostate cells.

Microscopy research and technique·2012

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Immunology

Background:

  • Catalytic antibodies are generated using stable analogues of transition states as haptens.
  • Current catalytic antibodies do not match the efficiency of natural enzymes.
  • This limitation necessitates a deeper understanding of transition state structures.

Purpose of the Study:

  • To analyze transition state structures in detail.
  • To propose novel strategies for designing stable transition state analogues.
  • To enhance the catalytic efficiency of elicited antibodies.

Main Methods:

  • Computational analysis of transition state structures.
  • Design and synthesis of novel stable analogues using atom substitution, insertion, and double atom insertion.
  • Application of these analogues to Diels-Alder and addition-elimination reactions.
  • Exploration of expanded transition states to prevent product inhibition.

Main Results:

  • Transition states often exhibit longer interatomic distances than previously used analogues.
  • Proposed methods (atom substitution, insertion, double atom insertion) offer solutions for synthesizing improved analogues.
  • The concept of expanded transition states shows promise for associative processes.

Conclusions:

  • Improved transition state analogue design is crucial for developing highly efficient catalytic antibodies.
  • Novel synthetic strategies can overcome limitations in current antibody catalysis.
  • Expanded transition states offer a potential solution to product inhibition in antibody catalysis.

Related Experiment Videos