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Cyclooxygenase-2 immunoreactivity in collagenous colitis.
Signe Wildt1, Jüri J Rumessen, Claudio Csillag
1Department of Medical Gastroenterology F, Gentofte Hospital and University of Copenhagen, Denmark. siw@dadlnet.dk
Collagenous colitis (CC) involves increased cyclo-oxygenase-2 (COX-2) in the colon, primarily in macrophages. This suggests COX-2 and prostaglandins play a role in CC inflammation.
Area of Science:
- Gastroenterology
- Immunology
- Inflammatory Bowel Disease Research
Background:
- Collagenous colitis (CC) is an inflammatory bowel disease with unknown causes.
- Prostaglandins and cyclo-oxygenase-2 (COX-2) are implicated in ulcerative colitis and Crohn's disease pathogenesis.
Purpose of the Study:
- To investigate the presence and location of COX-2 in the colonic mucosa of CC patients.
- To compare COX-2 expression in CC with normal controls and other inflammatory bowel diseases.
Main Methods:
- Immunohistochemistry, immunofluorescence, and Western blot analysis were used.
- Colonic biopsies from CC patients, normal controls, and ulcerative colitis/Crohn's disease patients were analyzed.
Main Results:
- Collagenous colitis patients showed increased COX-2 protein expression compared to controls.
- COX-2 was localized to mononuclear cells in the lamina propria, particularly macrophages.
- COX-2 expression in CC was similar to that in ulcerative colitis and Crohn's disease.
Conclusions:
- Increased COX-2 expression, mainly in macrophages, is a feature of collagenous colitis.
- Enhanced macrophage recruitment and COX-2 expression may contribute to CC pathogenesis via prostaglandin synthesis.
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