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Crystallographic data for chicken liver fructose bisphosphatase
The Journal of Biological Chemistry
|August 10, 1977
Summary
Researchers successfully grew large, single crystals of fructose bisphosphatase. Crystallographic analysis determined the crystal structure, including space group and cell dimensions, revealing one tetramer per asymmetric unit.
Area of Science:
- Biochemistry
- Structural Biology
- Crystallography
Background:
- Fructose bisphosphatase is a key enzyme in gluconeogenesis.
- Understanding its structure is crucial for metabolic research.
Purpose of the Study:
- To obtain high-quality crystals of fructose bisphosphatase for structural analysis.
- To determine the preliminary crystallographic parameters of the enzyme.
Main Methods:
- Crystal growth under various conditions.
- Preliminary X-ray crystallographic analysis.
Main Results:
- Large, single crystals of fructose bisphosphatase were obtained.
- The space group was identified as R3.
- Hexagonal cell dimensions: a = b = 304 Å, c = 80.4 Å.
- One tetramer was found per asymmetric unit.
Conclusions:
- The study provides essential crystallographic data for fructose bisphosphatase.
- These findings pave the way for detailed structural studies of the enzyme.