Potential agents to control enamel caries-like lesions
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Updated: Jun 21, 2026

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
F C Rehder Neto1, F A Maeda, C P Turssi
1Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Objectives:
To assess whether pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and calcium sodium phosphosilicate (CSP) control artificial caries lesion progression.
Methods:
Enamel slabs of bovine teeth were embedded in polyester resin and had their enamel surfaces serially polished, and tested for surface microhardness (SMH, 25 g, 5 s). Incipient caries-like lesions were pre-formed and specimens were evaluated by microhardness test (SMH(post-lesion)) and randomly assigned to five treatment groups (n=15): (1) regular dentifrice (RE, 1,100 ppm F); (2) dentifrice with calcium sodium phosphosilicate (CSP); (3) amorphous calcium phosphate stabilized by casein phosphopeptide (CPP-ACP); (4) CPP-ACP with 900 ppm F (CPP-ACP+F) and (5) control group-unexposed to any remineralizing agent. Treatments were applied five times, after the de-remineralization period in the cariogenic challenges. Post-treatments SMH measurements were conducted (SMH(post-treatment)).
Results:
ANOVA was applied for data evaluation and revealed a significant difference among the treatments (p=0.0161). Tukey's test was conducted and the percentage of mineral loss was calculated. Specimens exposed to CSP (7.1%), RE (6.7%) and CPP-ACP+F (3.8%) showed lower mineral loss than those that in the control group (-11.0%). CPP-ACP group (3.2%) differed from the control, CSP and RE groups.
Conclusions:
Depending on the agent used, a remineralizing effect may be expected, which reflects in caries lesions progression.
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