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Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jun 21, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Fully automated protein purification.

DeMarco V Camper1, Ronald E Viola

  • 1Department of Chemistry, University of Toledo, Toledo, OH 43606, USA.

Analytical Biochemistry
|July 15, 2009
PubMed
Summary

Automated protocols were developed to streamline protein purification, reducing manual labor and saving time. These methods enable flexible, fully automated protein purification using various chromatography techniques with minimal user intervention.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • High-purity proteins are crucial for structural and functional studies.
  • Traditional protein purification involves multiple steps (capture, intermediate, polishing) that are time-consuming and labor-intensive.
  • Minimizing operator intervention is key to improving efficiency and reproducibility.

Purpose of the Study:

  • To design and test automated protocols for protein purification.
  • To reduce hands-on time and operator dependency in protein purification workflows.
  • To create flexible and fully automated purification systems.

Main Methods:

  • Development of separate automated methods for sample loading, column washing, elution, and peak collection.
  • Integration of automated steps for ion exchange, metal affinity, hydrophobic interaction, and gel filtration chromatography.

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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

Related Experiment Videos

Last Updated: Jun 21, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

  • Sequential coupling of automated methods for flexible, multi-step purification.

    • Main Results:

    • Successfully automated individual purification steps across multiple chromatography types.
    • Demonstrated the ability to couple these automated methods sequentially.
    • Achieved protein purification with minimal operator intervention.

    Conclusions:

    • Automated protocols significantly enhance the efficiency of protein purification.
    • The developed system offers flexibility and can be adapted to various purification strategies.
    • This approach paves the way for fully automated, high-throughput protein purification.