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Some electrospray mass spectrometric evidence for the existence of covalent O-acyl enzyme intermediates
D S Ashton1, C R Beddell, D J Cooper
1Wellcome Research Laboratories, Beckenham, Kent, UK.
FEBS Letters
|November 4, 1991
Abstract:
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (alpha-chymotrypsin, subtilisin Carlsberg and subtilisin BPN') before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O-acyl enzyme intermediates had been formed.