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Mutational analysis of the HTLV-I trans-activator, Tax
A Heder1, S Paca-Uccaralertkun, I Boros
1Institute of Biochemistry, Biological Research Center, Szeged, Hungary.
FEBS Letters
|November 4, 1991
Abstract:
The gene coding for the trans-activating factor (Tax) of the human T-cell leukemia virus, type I (HTLV-I) was mutagenized in vitro using oligonucleotide-directed mutagenesis and recombinant DNA techniques. All except one of the mutagenized tax constructs failed to trans-activate the HTLV-I LTR in a eukaryotic test system. Moreover, negative Tax mutant Arg-39----Gly was found to be trans-dominant. This observation suggests that Tax contains distinct functional domains mediating different interactions of the protein in the process of trans-activation.