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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
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Published on: December 25, 2015

The inoculum effect on the ammonia-oxidizing bacterial communities in parallel sequential batch reactors.

Lieven Wittebolle1, Willy Verstraete, Nico Boon

  • 1Laboratory of Microbial Ecology & Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Gent, Belgium.

Water Research
|July 15, 2009
PubMed
Summary
This summary is machine-generated.

Wastewater treatment reactors seeded with different sludges maintained high nitrification but distinct microbial communities. Community biodiversity and dynamics, not specific species, indicate good microbial functionality.

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Area of Science:

  • Environmental microbiology
  • Wastewater treatment technologies

Background:

  • Sequential batch reactors (SBRs) are crucial for wastewater treatment.
  • Understanding microbial community structure and function is key to optimizing WWTP performance.

Purpose of the Study:

  • To investigate the phylogenetic similarity and functional stability of microbial communities in SBRs inoculated with diverse wastewater sludge.
  • To determine if distinct sludge origins lead to divergent or converging microbial communities and functionality over time.

Main Methods:

  • Parallel operation of three identical SBRs using sludge from hospital, meat processing, and municipal WWTPs for 84 days.
  • Analysis of total bacterial and ammonia-oxidizing bacterial (AOB) communities using PCR-DGGE.
  • Calculation of community characteristics including range-weighted richness and Pareto-Lorenz evenness distribution.

Main Results:

  • All SBRs exhibited high nitrification functionality and similar sludge floc characteristics.
  • Distinct bacterial communities were observed across the three SBRs, with no trend towards similarity.
  • Comparable internal structure and dynamics of AOB communities were found despite minimal shared species.

Conclusions:

  • Microbial community composition in SBRs remains distinct based on sludge origin.
  • Community biodiversity and dynamics are more critical indicators of microbial functionality than the presence of specific bacterial species.