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A model for fd phage penetration and assembly.

A K Dunker1, L D Ensign, G E Arnold

  • 1Department of Biochemistry and Biophysics, Washington State University, Pullman 99164.

FEBS Letters
|November 4, 1991
PubMed
Summary
This summary is machine-generated.

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Chloroform induces temperature-dependent structural changes in fd phage, forming compact I-forms and S-forms. These transformations mimic key steps in viral penetration and assembly processes.

Area of Science:

  • Structural biology
  • Virology
  • Biophysics

Background:

  • Fd phage is a filamentous bacteriophage.
  • Chloroform induces structural changes in fd phage at different temperatures.

Purpose of the Study:

  • To investigate the structural changes in fd phage induced by chloroform at varying temperatures.
  • To elucidate the relationship between these structural changes and viral processes like penetration and assembly.

Main Methods:

  • Treatment of fd phage with chloroform at temperatures below and above 15 degrees C.
  • Analysis of coat protein structure in native, I-form, and S-form fd phage.

Main Results:

  • Below 15°C, chloroform induced fd phage to contract into I-forms (1/3 length).

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  • Above 15°C, I-forms further contracted into spheroidal S-forms.
  • I-form coat protein structure matches native phage; S-form structure matches protein in bilayers.

    • Conclusions:

    • Fd phage structural conversions mimic steps in viral penetration.
    • Reverse conversions mimic steps in viral assembly.