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Solubilization and partial purification of squalene synthase from daffodil microsomal membranes
L Belingheri1, P Beyer, H Kleinig
1Laboratoire de Physiologie Cellulaire Végétale, Université de Bordeaux, Talence, France.
FEBS Letters
|November 4, 1991
Abstract:
A squalene synthase was solubilized from daffodil (Narcissus pseudonarcissus L.) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was prepared.