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A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
Published on: July 6, 2017
MYC insertions in diffuse-type gastric adenocarcinoma.
Danielle Queiroz Calcagno1, Adriana Costa Guimarães, Mariana Ferreira Leal
1Human Cytogenetics Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.
Anticancer Research
|July 15, 2009
Summary
MYC gene alterations, including copy number changes and insertions, are frequent in diffuse-type gastric cancer. Cytoplasmic MYC expression, linked to these genetic events, may be a common feature of this cancer type.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The MYC oncogene plays a critical role in gastric carcinogenesis.
- Previous research has indicated associations between MYC translocations/insertions and gastric cancer development.
Purpose of the Study:
- To investigate MYC copy number variations, gene insertions, and protein expression in diffuse-type gastric cancer.
- To explore the relationship between MYC genetic alterations and its cellular localization.
Main Methods:
- Fluorescence in situ hybridization (FISH) was employed to assess MYC copy number and insertion sites in 12 diffuse-type gastric cancer samples.
- Immunohistochemistry was utilized to evaluate MYC protein expression levels and localization.
Main Results:
- Increased MYC copy numbers (3, 4, and 5 signals) were observed in all samples.
- Chromosome 8 trisomy and MYC insertions into chromosomes 2, 7, 14, 17, 18, and 22 were detected.
- Both nuclear and cytoplasmic MYC immunoreactivity were present in all analyzed gastric cancer tissues.
Conclusions:
- MYC cytoplasmic immunoreactivity potentially arises from gene insertions near nuclear-targeting regions.
- MYC insertion and subsequent cytoplasmic expression may represent a common molecular characteristic of diffuse-type gastric cancer.
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