Metabolic network analysis integrated with transcript verification for sequenced genomes
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Updated: Jun 21, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
Ani Manichaikul1, Lila Ghamsari, Erik F Y Hom
1Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Abstract:
With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering.
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