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Microarray Analysis for Saccharomyces cerevisiae
Published on: April 7, 2011
Vanadium pentoxide effects on stress responses in wine Saccharomyces cerevisiae strain UE-ME3
Tânia Rosado1, Ana Conim, Isabel Alves-Pereira
1Departamento de Química, Universidade de Evora, R.Romão Ramalho, 59, 7002-671, Evora, Portugal.
Abstract:
Vanadium pentoxide mainly used as catalyst in sulphuric acid, maleic anhydride and ceramics industry, is a pollutant watering redistributed around the environment. Research on biological influence of vanadium pentoxide has gained major importance because it exerts toxic effects on a wide variety of biological systems. In this work we intent to evaluate the effects of vanadium pentoxide ranging from 0 to 2 mM in culture media on a wine wild-type Saccharomyces cerevisiae from Alentejo region of Portugal. Our results show that 2.0 mM vanadium pentoxide in culture medium induced a significant increase of malonaldehyde level and Glutathione peroxidase activity, a slightly increase of Catalase A activity as well as a decrease of wet weight and mitochondrial NADH cit c reductase of S. cerevisiae UE-ME(3). Also our results show that cycloheximide prevent cell death when cells grows 30 min in presence of 1.5 mM of vanadium pentoxide.
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