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High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
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Published on: February 9, 2019

Hydroxamate siderophores of Scedosporium apiospermum

Samuel Bertrand1, Gérald Larcher, Anne Landreau

  • 1Laboratoire des Substances Naturelles et Analogues Structuraux, UPRES-EA 921, IFR 149 QUASAV, UFR de Pharmacie et Ingénierie de Santé, Université Université d'Angers,16 Bd Daviers, 49000 Angers, France

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
|July 15, 2009
PubMed

Insights

Scedosporium apiospermum produces hydroxamate siderophores, including dimerumic acid and Nα-methyl coprogen B. Clinical respiratory isolates showed higher Nα-methyl coprogen B production, suggesting potential diagnostic use for scedosporiosis.

Area of Science:

  • Medical Mycology
  • Biochemistry

Background:

  • Scedosporium apiospermum is an emerging fungal pathogen.
  • It causes severe infections in immunocompromised patients and cystic fibrosis patients.

Purpose of the Study:

  • To investigate the pathogenic mechanisms of Scedosporium apiospermum.
  • To identify siderophores produced by the fungus.

Main Methods:

  • Cultivation on CAS medium and specific assays.
  • Iron-restricted liquid culture at alkaline pH.
  • Liquid/liquid extraction and HPLC separation.
  • Identification by ESI-MS and MS-MS fragmentation.

Main Results:

  • Hydroxamates were secreted, with maximal production under specific conditions.
  • Dimerumic acid and Nα-methyl coprogen B were identified.
  • Clinical respiratory isolates produced more Nα-methyl coprogen B.

Conclusions:

  • Scedosporium apiospermum produces specific siderophores.
  • Nα-methyl coprogen B may serve as a diagnostic marker for scedosporiosis.

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