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A monoclonal antibody to DNA modified with osmium tetroxide/2,2'-bipyridine
A E Kabakov1, V K Podgorodnichenko, A M Poverenny
1Institute of Medical Radiology, Academy of Medical Sciences, Obninsk, Kaluga Region, U.S.S.R.
Abstract:
A murine monoclonal antibody (IgG) has been generated that binds to DNA modified with osmium tetroxide in the presence of 2,2'-bipyridine and does not interact with unmodified DNA. Reactivity of the antibody was tested by gel retardation assay, ELISA, dot-binding assay and immunoblotting. The results obtained suggest that the antibody does not cross-react with modified or unmodified RNA or proteins. The high specificity of the binding reaction is due to the specific recognition of modified deoxythymidine residue by the monoclonal antibody. A possible way of using the antibody produced is discussed.