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Updated: Jun 21, 2026

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Published on: April 20, 2016
Langerhans cell migration: not necessarily always at the center of the skin sensitization universe
Ian Kimber1, Marie Cumberbatch, Rebecca J Dearman
1Faculty of Life Sciences, University of Manchester, Manchester, UK. ian.kimber@manchester.ac.uk
Abstract:
Since their discovery in 1868, the role of Langerhans cells (LCs) in skin immunity has been researched extensively. Recent data deriving from transgenic animals that are deficient in LCs have begun to challenge the dogma that there is a universal requirement for these cells in the development of skin sensitization. This Commentary addresses relationships between LC mobilization, draining lymph node activation, and skin sensitization using immunomodulators agonistic for a family of sphingosine-1-phosphate (S1P) receptors.
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