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Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...

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Updated: Jun 21, 2026

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
13:10

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

Flii control: balancing migration and adhesion.

Kristina Kligys1, Jonathan C R Jones

  • 1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

The Journal of Investigative Dermatology
|July 16, 2009
PubMed
Summary
This summary is machine-generated.

The actin-remodeling protein Flii impacts skin wound healing. Its overexpression hinders healing and hemidesmosome formation, while deficiency enhances healing and promotes adhesion.

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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
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Area of Science:

  • Cell biology
  • Dermatology
  • Biochemistry

Background:

  • Skin wound healing necessitates a balance between cell adhesion and migration.
  • These cellular processes involve dynamic regulation of the cytoskeleton, cell-surface receptors, and their ligands.

Purpose of the Study:

  • To investigate the role of the actin-remodeling protein Flii in skin wound healing.
  • To elucidate the mechanisms by which Flii influences keratinocyte adhesion and motility.

Main Methods:

  • Overexpression and deficiency models of Flii in keratinocytes.
  • Assessment of wound healing rates.
  • Analysis of hemidesmosome formation and assembly.
  • Investigation of cytoskeletal dynamics and cell motility.

Main Results:

  • Overexpression of Flii impeded wound healing and inhibited hemidesmosome formation.
  • Flii deficiency resulted in enhanced wound healing.
  • Flii deficiency promoted hemidesmosome assembly, suggesting a role in stable keratinocyte adhesion.

Conclusions:

  • Flii plays a critical role in regulating the balance between keratinocyte adhesion and migration during wound healing.
  • The gelsolin family member Flii may act as a key regulator of stable keratinocyte adhesion and motility.
  • Modulating Flii activity could offer therapeutic strategies for improving wound healing.