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Published on: May 22, 2014
Detection and characterization of soluble CD93 released during inflammation
Mallary C Greenlee1, Sarah A Sullivan, Suzanne Slater Bohlson
1Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, IN 46556, USA. mgreenle@nd.edu
Summary
Inflammation increases soluble CD93 (sCD93) levels, primarily released by macrophages. This soluble form enhances the clearance of apoptotic cells, revealing a key mechanism in immune response.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD93 is a cell surface glycoprotein crucial for apoptotic cell engulfment.
- Soluble CD93 (sCD93) is found in human plasma, released from activated immune cells.
Purpose of the Study:
- To investigate the link between sCD93 production, inflammation, and apoptotic cell clearance.
- To understand the role of inflammation in regulating sCD93 levels and function.
Main Methods:
- Induction of sterile peritonitis in mice to study inflammation.
- Analysis of peritoneal lavage fluid for sCD93 using ELISA and Western blot.
- Assessment of cellular infiltrate and their CD93 shedding capacity in vitro.
Main Results:
- Peritonitis led to an 8.9-fold increase in sCD93.
- Macrophages were the primary leukocytes during peak sCD93 levels.
- Inflammatory macrophages shed CD93 in vitro, contributing to in vivo levels.
- sCD93 in inflammatory fluid enhanced apoptotic cell engulfment.
Conclusions:
- Inflammation triggers in vivo release of sCD93.
- Inflammatory macrophages are a significant source of sCD93.
- sCD93 plays a role in facilitating the engulfment of apoptotic cells.
