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Targeted optical injection of gold nanoparticles into single mammalian cells

Craig McDougall1, David J Stevenson, Christian T A Brown

  • 1SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK. cm70@st-andrews.ac.uk

Journal of Biophotonics
|July 16, 2009
PubMed

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.