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Updated: May 23, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Transmural flow bioreactor for vascular tissue engineering.

Jason W Bjork¹, Robert T Tranquillo

¹Department of Biomedical Engineering, University of Minnesota 55455, USA.

Biotechnology and Bioengineering

|July 16, 2009

Summary

This summary is machine-generated.

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Perfusion bioreactor configurations improve nutrient delivery for engineered tissues. Combining transmural and axial flow optimizes oxygen distribution, enhancing cell growth and uniformity in tissue-engineered arteries.

Area of Science:

Biomedical Engineering
Tissue Engineering
Bioreactor Design

Background:

Nutrient transport is a key limitation in culturing engineered tissues in vitro.
Developing functional tissue-engineered arteries requires uniform nutrient and oxygen delivery.

Purpose of the Study:

To investigate perfusion bioreactor configurations for uniform oxygen delivery to media equivalents (MEs).
To model and analyze different flow configurations for optimal oxygen profiles in tissue engineering.

Main Methods:

Evaluated transmural flow, axial flow, and combined flow configurations in bioreactors.
Developed transport models incorporating tissue hydraulic conductivity, cellular oxygen consumption, and cell density.
Verified model predictions with measured dissolved oxygen (DO) concentrations.

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Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor

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Last Updated: May 23, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

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Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor

Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor

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Main Results:

Combined transmural and axial flow significantly improved dissolved oxygen (DO) uniformity.
Identified potential detrimental effects like exceeding burst pressure or shear stress.
Model results were validated by experimental DO measurements.

Conclusions:

A combination of controlled transmural and axial flow enhances nutrient transport to cells.
This approach improves cell growth, extracellular matrix production, and uniformity in engineered tissues.
Optimized bioreactor design is crucial for successful tissue engineering applications.