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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Bacterial Gene Expression Analysis Using Microarrays

Bacterial Gene Expression Analysis Using Microarrays

Published on: May 28, 2007

Prokaryotic real-time gene expression profiling for toxicity assessment.

Annalisa Onnis-Hayden¹, Haifeng Weng, Miao He

¹Department of Civil and Environmental Engineering, Northeastern University, Boston, Massachusetts 02115, USA.

Environmental Science & Technology

|July 17, 2009

Summary

This study used real-time gene expression profiling in Escherichia coli to assess environmental toxins. It revealed distinct gene expression patterns for mercury and mitomycin, aiding toxicity mechanism understanding.

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Bacterial Gene Expression Analysis Using Microarrays

Bacterial Gene Expression Analysis Using Microarrays

Published on: May 28, 2007

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

Area of Science:

Environmental toxicology
Molecular biology
Genomics

Background:

Global gene expression profiling is a powerful tool for toxicity assessment.
Understanding toxin mechanisms requires detailed analysis of gene expression changes.

Purpose of the Study:

To demonstrate the application of prokaryotic real-time gene expression profiling for environmental pollutant toxicity assessment.
To investigate the time-dependent transcriptional responses to mercury and mitomycin (MMC) in Escherichia coli.

Main Methods:

Utilized a cell-array library of 93 E. coli K12 strains with transcriptional green fluorescent protein (GFP) fusions.
Employed high-temporal-resolution gene expression profiling to monitor stress response genes.
Performed gene clustering analysis based on temporal response patterns.

Main Results:

Obtained compound-specific and concentration-dependent gene expression profiles for mercury and MMC.
MMC (a genotoxin) induced the SOS response and genes related to drug resistance and detoxification.
Identified mercury resistance genes, including zntA, which showed increased upregulation with higher mercury concentrations.

Conclusions:

Real-time gene expression profiling provides efficient and informative toxicity assessment for environmental samples.
This method reveals complex, time-dependent gene activities, offering mechanism-based toxicological insights.
Distinctive transcriptional profiles aid in identifying and characterizing the effects of specific environmental pollutants.