Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multiple Sclerosis l: Introduction01:19

Multiple Sclerosis l: Introduction

Multiple sclerosis is a chronic autoimmune disease of the central nervous system (CNS) that affects the brain, spinal cord, and optic nerves. It is an inflammatory demyelinating disorder and a leading cause of neurological disability in young adults.EpidemiologyMS commonly begins between 20 and 40 years of age and is twice as common in women. Its exact cause remains unclear, but genetic susceptibility contributes, with higher risk in first-degree relatives and identical twins. A greater...
Glial Cells01:04

Glial Cells

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of in vitro hyperthermia on fatty acids of red blood cells and plasma lipids from patients with multiple sclerosis.

Journal of the neurological sciences·1990
Same author

Can the hypotriglyceridemic effect of fish oil concentrate be sustained?

Annals of internal medicine·1989
Same author

Oligoclonal bands in serum of patients with multiple sclerosis.

Clinical chemistry·1988
Same author

Sialic acid and fatty acid concentrations in lymphocytes, red blood cells and plasma from patients with multiple sclerosis.

Journal of the neurological sciences·1984
Same author

Abnormal lipid composition of fat tissue in human mesenteric panniculitis.

Lipids·1981
Same author

Amniotic fluid lecithin/sphingomyelin ratio changes related to centrifugal force.

Obstetrics and gynecology·1977

Related Experiment Video

Updated: Jun 21, 2026

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

Plasma gangliosides in multiple sclerosis.

M M Lebrun1, G D Cherayil

  • 1Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wisc. 53226, USA.

Neuroscience Letters
|July 17, 2009
PubMed
Summary

Multiple sclerosis (MS) patients have significantly lower total plasma ganglioside levels compared to controls. The major ganglioside, GM(3), showed no compositional differences in fatty acids or hexoses between groups.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Clinical Chemistry

Background:

  • Gangliosides are complex glycosphingolipids crucial for neuronal function.
  • Alterations in ganglioside metabolism are implicated in various neurological disorders.
  • Multiple Sclerosis (MS) is a chronic inflammatory disease of the central nervous system.

Purpose of the Study:

  • To investigate the total concentration and relative distribution of plasma gangliosides in patients with multiple sclerosis (MS).
  • To analyze the fatty acid and hexose constituents of the major plasma ganglioside, GM(3), in MS patients and controls.

Main Methods:

  • Plasma gangliosides were isolated and quantified using N-acetylneuraminic acid (sialic acid) measurements.
  • Gas-liquid chromatography was employed to determine the fatty acid composition of GM(3).

More Related Videos

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
08:36

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Published on: March 23, 2017

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
10:00

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Published on: November 8, 2016

Related Experiment Videos

Last Updated: Jun 21, 2026

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
08:36

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Published on: March 23, 2017

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
10:00

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Published on: November 8, 2016

  • Hexose moieties of GM(3) were analyzed in the MS group.

    • Main Results:

    • GM(3) was identified as the predominant plasma ganglioside in both MS patients and control subjects.
    • Total plasma ganglioside concentration was significantly reduced in the MS group compared to controls.
    • No significant differences were found in the distribution of ganglioside fractions, GM(3) fatty acid composition, or GM(3) hexose composition (glucose and galactose in a 1:1 ratio) between the groups.

    Conclusions:

    • The study identifies a significant decrease in total plasma ganglioside levels in individuals with multiple sclerosis.
    • Despite reduced overall levels, the composition of the major ganglioside GM(3) remains largely unchanged in MS patients.
    • These findings suggest that altered ganglioside levels, rather than compositional changes in GM(3), may be relevant in the pathophysiology of MS.