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Updated: Jun 21, 2026

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
Published on: May 4, 2022
Actin-like filaments in synaptosomes detected by heavy meromyosin binding
N C Inestrosa1, H L Fernandez, J Garrido
1Department of Cell Biology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile; Laboratory of Neurophysiology, Catholic University of Chile, P.O. Box 114-D, Santiago Chile.
Abstract:
We have prepared cat forebrain synaptosomes in order to investigate the presence of actin-like proteins. First, actin-like filaments were demonstrated using a heavy meromyosin binding technique. Decorated filaments form networks inside the synaptosomes and are frequently seen in relation to membranes and synaptic vesicles. Second, using SDS-polyacrylamide gel electrophoresis, a band corresponding to actin appears to be one of the major constituents of synaptosomal fractions; this confirms the work of Fine and his coworkers. The significance of actin-like filaments in relation to axoplasmic transport is briefly discussed.
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