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Related Experiment Video

Updated: Jun 21, 2026

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
10:00

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Published on: January 12, 2015

In vitro protein synthesis by oligodendroglial cells.

N L Banik1, M E Smith

  • 1Department of Neurology, Veterans Administration Hospital, Palo Alto U.S.A.

Neuroscience Letters
|July 17, 2009
PubMed
Summary

Researchers isolated rat oligodendroglial cells and found they synthesize protein. This protein synthesis, measured by [U-(14)C]leucine incorporation, was confirmed to be an active cellular process inhibited by specific drugs.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Oligodendroglial cells are crucial for central nervous system myelination.
  • Understanding their metabolic activity is key to studying myelin maintenance and repair.

Purpose of the Study:

  • To isolate and characterize the protein synthesis capabilities of rat oligodendroglial cells.
  • To investigate the metabolic activity of isolated oligodendroglial cells.

Main Methods:

  • Oligodendroglial cells were isolated from rat spinal cord and brain stem using a modified Poduslo and Norton method.
  • Protein synthesis was assessed by measuring the incorporation of [U-(14)C]leucine into trichloroacetic acid (TCA)-insoluble protein.
  • Cell purity was verified using phase contrast microscopy and 2',3'-cyclic nucleotide phosphohydrolase (CNPase) activity assays.

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Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
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Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Published on: February 17, 2016

Related Experiment Videos

Last Updated: Jun 21, 2026

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
10:00

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Published on: January 12, 2015

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
10:53

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
11:11

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Published on: February 17, 2016

Main Results:

  • Isolated oligodendroglial cells demonstrated active incorporation of [U-(14)C]leucine into protein over 120 minutes.
  • This leucine incorporation, indicative of active protein synthesis, was significantly inhibited by cycloheximide and chloramphenicol.
  • The cell preparation achieved high purity, confirmed by microscopy and CNPase activity.

Conclusions:

  • Rat oligodendroglial cells possess the capacity for active protein synthesis.
  • The observed protein synthesis is an energy-dependent process sensitive to specific inhibitors.
  • This study provides a foundation for further research into oligodendroglial cell metabolism and function.