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Calcium decrease associated with aminopyridine-induced potassium increase in cat cerebellum.

C Nicholson1, R Steinberg, H Stöckle

  • 1Physiological Institute, University of München, 8 München 2 G.F.R.

Neuroscience Letters
|July 17, 2009
PubMed
Summary
This summary is machine-generated.

Aminopyridine superfusion in cat cerebellum caused significant extracellular calcium (Ca2+) decrease and potassium (K+) increase. These ion changes, localized to active regions, suggest aminopyridine enhances Ca2+ membrane currents.

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Area of Science:

  • Neuroscience
  • Neurophysiology

Background:

  • Extracellular ion fluctuations are critical for neuronal function.
  • Aminopyridine is known to affect neuronal excitability.

Purpose of the Study:

  • To investigate the effects of aminopyridine on extracellular Ca(2+) and K(+) in the cat cerebellum.
  • To characterize the spatial distribution and reversibility of these ion changes.

Main Methods:

  • Utilized Ca(2+)- and K(+)-selective micropipettes for simultaneous extracellular ion measurements.
  • Superfused cat cerebellum with aminopyridine-Ringer solution.
  • Applied a single shock to the cerebellar surface and observed ion transients.

Main Results:

  • A single cerebellar shock induced a transient decrease in Ca(2+) and an increase in K(+).
  • The Ca(2+) decrease was spatially localized to the synaptically active region.
  • These ion changes were reversible upon addition of citrate to the superfusate.

Conclusions:

  • Aminopyridine superfusion in the cerebellum leads to significant extracellular ion transients.
  • The observed ion dynamics resemble those seen in spreading depression.
  • Results suggest aminopyridine may enhance voltage-gated Ca(2+) membrane currents.