Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The MCP-4/MCP-1 ratio in plasma is a candidate circadian biomarker for chronic post-traumatic stress disorder.

Translational psychiatry·2017

Same author

Localization of α7 nicotinic acetylcholine receptor mRNA and protein within the cholinergic anti-inflammatory pathway.

Neuroscience·2014

Same author

Immunocytochemical study of parvalbumin, calbindin D-28k, and calretinin in the superficial dorsal horn of the rat spinal cord following unilateral hindpaw inflammation.

Journal of anesthesia·2013

Same author

Development of cardiac parasympathetic neurons, glial cells, and regional cholinergic innervation of the mouse heart.

Neuroscience·2012

Same author

Sympathetic innervation and chemical sympathectomy of canine bladder.

Urology·2011

Same author

Identification of a calcium binding protein highly localized to the cochlear nucleus.

Neurochemistry international·2010

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

A method for sectioning microwave-fixed brain prior to microdissection and acetylcholine analysis.

D B Hoover¹, E A Muth, D M Jacobowitz

¹Laboratory of Clinical Science, National Institute of Mental Health, Building 10/Room 2D-46, Bethesda, MD 20014, USA.

Neuroscience Letters

|July 17, 2009

Summary

A new vibratome sectioning method using FC-47 liquid preserves acetylcholine (ACh) distribution in rat brain sections, unlike cryostat or buffer methods.

More Related Videos

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

Related Experiment Videos

Last Updated: Jun 21, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Published on: October 7, 2021

Area of Science:

Neuroscience
Biochemistry

Background:

Accurate measurement of neurotransmitter distribution in the brain requires high-quality tissue sections.
Previous methods like cryostat sectioning of microwave-irradiated rat brains yielded poor quality sections unsuitable for microdissection.

Purpose of the Study:

To develop and evaluate a reliable method for preparing 300 micrometer rat brain sections for microdissection and subsequent analysis of labile substances like acetylcholine (ACh).

Main Methods:

Comparison of cryostat sectioning versus vibratome sectioning of unfrozen rat brains.
Evaluation of different immersion liquids for vibratome sectioning: Krebs-Ringer buffer and an inert fluorocarbon liquid (FC-47).
Measurement of acetylcholine (ACh) diffusion from brain sections prepared using different methods.

Main Results:

Cryostat sections exhibited rough surfaces and cracks, compromising tissue integrity.
Vibratome sectioning in Krebs-Ringer buffer led to apparent diffusion of ACh from the tissue.
Vibratome sectioning in FC-47 resulted in smooth, intact sections with no detectable diffusion of ACh.

Conclusions:

Vibratome sectioning of unfrozen rat brains immersed in FC-47 is a superior method for preserving the distribution of labile substances like ACh.
This technique facilitates accurate microdissection and subsequent analysis of neurotransmitter distribution in the rat brain.