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Published on: August 16, 2018
Benzodiazepine receptor binding in rat spinal cord membranes
W E Müller1, U Schläfer, U Wollert
1Pharmakologisches Institut der Universität Mainz, Obere Zahlbacher Strasse 67, D-6500 Mainz, G.F.R.
Abstract:
Using [(3)H]diazepam, benzodiazepine receptor binding has been investigated in rat spinal cord synaptosomal membranes. Specific [(3)H]diazepam binding is saturable with a K(D) of about 8 nM and a maximal number of binding sites of 0.48 pmol/mg protein, which is about half of the density found in rat brain synaptosomal membranes. Using 5 benzodiazepine derivatives and amino acid neurotransmitters and their antagonists, no differences in specific [(3)H]diazepam binding to rat spinal cord and rat brain synaptosomal membranes were found. The results do not support the hypothesis that benzodiazepines act via glycine receptors in the spinal cord.
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