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Updated: Jun 21, 2026

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Published on: March 12, 2019
ACTH controls [(3)H]dopamine uptake in the adrenal chromaffin cell
1Departments of Physiology and Anatomy, Niigata University School of Medicin, Niigata Japan.
Abstract:
Effect of hypophysectomy on the disposition of [(3)H]dopamine-derived radioactivity in the mouse adrenal medulla was examined by autoradiography. Chromaffin cells, both A and NA type, incorporated less radioactivity in the hypophysectomized mice (50 days after operation) than in normal control mice: this decrease in the [(3)H]dopamine uptake was restored by intraperitoneal injection of ACTH (for 26 days; starting from 24th day after hypophysectomy; 40 mug/gbw/day). In the control mice chromaffin cells near the cortico-medullary junction showed a higher radioactivity than those in the center: hypophysectomy obscured this specific distribution pattern which reappeared after ACTH treatment.
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