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Role of annexin A2 in cellular entry of rabbit vesivirus

Salomé González-Reyes1, Alberto García-Manso1, Gloria Del Barrio1

  • 1Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain.

The Journal of General Virology
|July 17, 2009
PubMed

Insights

Rabbit vesivirus (RaV) uses annexin A2 (ANXA2) for cell attachment and entry. Blocking ANXA2 with antibodies or RNA interference significantly reduced RaV infection, highlighting ANXA2

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Calicivirus attachment and internalization mechanisms remain poorly understood due to limited cell culture systems.
  • Rabbit vesivirus (RaV) is a member of the Caliciviridae family.

Purpose of the Study:

  • To investigate the cellular mechanisms of RaV attachment and internalization.
  • To identify host cell receptors involved in RaV infection.

Main Methods:

  • Virus overlay protein-binding assay using HEK293T cell membrane fractions.
  • Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis.
  • Monoclonal anti-ANXA2 antibody neutralization.
  • Small interfering RNA (siRNA)-mediated knockdown of ANXA2 expression.

Main Results:

  • RaV virions specifically bind to annexin A2 (ANXA2).
  • ANXA2 was identified as a binding partner using biochemical assays.
  • Inhibition of ANXA2 function (via antibody or siRNA) significantly reduced RaV infection.

Conclusions:

  • Annexin A2 (ANXA2) is a key host factor involved in rabbit vesivirus (RaV) attachment and/or internalization.
  • This study provides a foundation for understanding calicivirus-host interactions.

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