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Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

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Related Experiment Video

Updated: Jun 21, 2026

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

Single-antigen serological testing for bovine tuberculosis.

Lawrence R Green¹, Cynthia C Jones, Anne L Sherwood

¹PriTest Inc., Redmond, WA 98052, USA.

Clinical and Vaccine Immunology : CVI

|July 17, 2009

Summary

This summary is machine-generated.

A new single-antigen test using MPB83 detects Mycobacterium bovis infection in cattle. This chemiluminescent assay shows high sensitivity and specificity, offering a cost-effective alternative for bovine tuberculosis diagnosis.

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Last Updated: Jun 21, 2026

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Published on: June 15, 2018

Area of Science:

Veterinary Immunology
Bacteriology
Diagnostic Assays

Background:

Antibody responses are key indicators of Mycobacterium bovis infection in cattle.
Current multi-antigen tests are costly and prone to false positives.
A sensitive, specific single-antigen test is needed for bovine tuberculosis diagnosis.

Purpose of the Study:

To evaluate the SeraLyte-Mbv system, a novel single-antigen test for Mycobacterium bovis infection.
To assess the sensitivity and specificity of the MPB83-based chemiluminescent assay.

Main Methods:

The SeraLyte-Mbv system was tested using sera from cattle experimentally infected with M. bovis and other Mycobacterium species.
Species specificity was assessed using sera from M. kansasii, M. avium subsp. paratuberculosis, and M. avium subsp. avium infected cattle.
Blinded testing was performed on known infected and uninfected cattle, followed by unblinded analysis of a larger cohort.

Main Results:

The SeraLyte-Mbv system demonstrated 89% sensitivity for M. bovis infection.
High specificity was observed, with 98% of 895 animals from tuberculosis-free states testing negative.
The test showed 100% sensitivity for M. bovis infection after three months post-infection.

Conclusions:

The SeraLyte-Mbv system is a feasible single-antigen diagnostic tool for bovine tuberculosis.
This MPB83-based assay offers a sensitive and specific alternative to multi-antigen testing.
The chemiluminescent platform provides analytical sensitivity for reliable M. bovis detection.