Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibrotic marrow limiting morphologic classification in MDS/MPN with SF3B1 mutation and thrombocytosis: diagnostic implications under the ICC 2022 framework.

Virchows Archiv : an international journal of pathology·2026
Same author

Genetic risk and immune dysregulation of classic Hodgkin lymphoma transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma: a multicentric study.

medRxiv : the preprint server for health sciences·2026
Same author

Transcriptome sequencing of Hodgkin lymphoma Hodgkin and Reed-Sternberg cells reveals escape from NK cell recognition and an unfolded protein response.

Blood cancer journal·2026
Same author

Interpretable multiple instance learning for hematologic diagnosis from peripheral blood smears.

Communications medicine·2026
Same author

Leveraging Kappa-Lambda Signatures in a Multistage Machine Learning Pipeline for B-Cell Lymphoma Detection by Flow Cytometry.

The American journal of pathology·2026
Same author

Accurate automated diagnosis of B-acute lymphoblastic leukemia using deep learning and flow cytometry.

Haematologica·2026

Related Experiment Video

Updated: Jun 21, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Optimization of plasma fluorogenic thrombin-generation assays.

Wayne L Chandler1, Mikhail Roshal

  • 1Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195, USA.

American Journal of Clinical Pathology
|July 17, 2009
PubMed
Summary
This summary is machine-generated.

Optimizing thrombin generation assays with lower sample volumes and analytic corrections improves accuracy. This enhanced assay effectively differentiates sepsis patients from healthy individuals using minimal tissue factor activator.

More Related Videos

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model
06:16

Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 21, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model
06:16

Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model

Published on: April 19, 2024

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Hematology

Background:

  • Thrombin generation assays are crucial for evaluating hemostasis.
  • Optimizing assay conditions is essential for accurate clinical interpretation.
  • Previous methods faced limitations in sensitivity and reproducibility.

Purpose of the Study:

  • To optimize fluorogenic thrombin generation assays for improved accuracy and efficiency.
  • To evaluate the impact of reduced sample volume and analytic corrections.
  • To assess the assay's ability to differentiate healthy subjects from sepsis patients.

Main Methods:

  • Optimization of sample volume, calibration strategies, and activation reagents.
  • Implementation of analytic corrections for fluorescence, thrombin-alpha(2)-macroglobulin activity, and hemolysis.
  • Use of a corn trypsin inhibitor to block contact activation.

Main Results:

  • Lower sample volumes (40 microL) enhanced thrombin activity recovery and reduced light absorbance interference.
  • Analytic corrections significantly improved assay accuracy.
  • The optimized assay with 0.6 pmol/L tissue factor activator effectively differentiated sepsis patients from controls with good reproducibility (CV: 4% within-run, 7% between-run).

Conclusions:

  • Optimized thrombin generation assays using reduced sample volumes and analytic corrections offer enhanced accuracy.
  • The optimized assay demonstrates clinical utility in distinguishing sepsis patients.
  • Further validation of this optimized assay in diverse clinical settings is warranted.