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Related Concept Videos

Protein Organization01:13

Protein Organization

Overview
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

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Related Experiment Video

Updated: May 12, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Systems biology: Untangling the protein web.

Nathan Blow

    Nature
    |July 17, 2009
    PubMed
    Summary

    Scientists mapped numerous cellular macromolecular interactions. However, understanding these complex networks and their functions remains a significant scientific challenge.

    Area of Science:

    • Cellular biology
    • Molecular interactions
    • Systems biology

    Background:

    • Thousands of individual macromolecular interactions within cells have been identified.
    • Understanding the collective behavior and functional significance of these interactions is crucial for comprehending cellular processes.

    Purpose of the Study:

    • To highlight the challenge of integrating known macromolecular interactions into comprehensive cellular networks.
    • To emphasize the ongoing need for methods to decipher the functional roles of these complex molecular networks.

    Main Methods:

    • Review of current research on macromolecular interaction mapping.
    • Analysis of the challenges in network construction and functional interpretation.

    Main Results:

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    Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
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    Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

    Published on: June 15, 2018

    JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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    JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

    Published on: October 19, 2021

    Related Experiment Videos

    Last Updated: May 12, 2026

    Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
    08:38

    Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

    Published on: March 3, 2015

    Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
    06:45

    Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

    Published on: June 15, 2018

    JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
    07:28

    JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

    Published on: October 19, 2021

    • A large dataset of individual macromolecular interactions exists.
    • The integration of these interactions into functional networks is not yet complete.

    Conclusions:

    • Despite extensive identification of molecular interactions, a significant gap remains in understanding their network-level organization and functional implications.
    • Further research is needed to develop robust methodologies for building and interpreting cellular interaction networks.