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The Microfluidic Probe: Operation and Use for Localized Surface Processing

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

High-throughput mode liquid microjunction surface sampling probe.

Gary J Van Berkel¹, Vilmos Kertesz, Richard C King

¹Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, USA. vanberkelgj@ornl.gov

Analytical Chemistry

|July 18, 2009

Summary

A new automated method simplifies surface sampling for liquid microjunction surface sampling probe/electrospray ionization mass spectrometry (LMJ-SSP/ESI-MS). This technique enhances high-throughput quantitative analysis by eliminating the need for precise probe-to-surface alignment for each spot.

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Area of Science:

Analytical Chemistry
Mass Spectrometry

Background:

Traditional LMJ-SSP/ESI-MS requires precise (<20 µm) probe-to-surface alignment for liquid microjunction formation.
Multiple spot sampling necessitates repeated, time-consuming alignment procedures.

Purpose of the Study:

To develop a simplified, automated spot sampling mode for LMJ-SSP/ESI-MS.
To improve the efficiency and throughput of surface sampling analysis.

Main Methods:

A novel sampling mode was implemented where the probe-to-surface distance was less critical (100-300 µm) and remained constant.
Liquid microjunction was formed by extending probe liquid to the surface, controlled by adjusting self-aspiration and pumped flow rates.
Analytes were aspirated into the probe upon increasing self-aspiration rate for subsequent electrospray ionization mass spectrometry.

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Main Results:

The new method eliminates the need for critical probe-to-surface distance adjustments between sampling spots.
Demonstrated potential for high-throughput quantitative analysis of surface-bound analytes.
Successful sampling and analysis of multiple spots without re-alignment.

Conclusions:

The simplified automated spot sampling mode significantly enhances the operational efficiency of LMJ-SSP/ESI-MS.
This advancement facilitates rapid, high-throughput quantitative analysis, making it suitable for various applications.