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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Microsphere-based tracing and molecular delivery in embryonic stem cells.

Anestis Tsakiridis1, Lois M Alexander, Nicole Gennet

  • 1MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JQ, UK.

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|July 18, 2009
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Summary

Researchers developed a novel microsphere system for efficient delivery of biological materials into embryonic stem (ES) cells. This method enables simultaneous labeling and cargo transfer without affecting cell pluripotency or viability.

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Area of Science:

  • Stem cell biology
  • Biotechnology
  • Cellular delivery systems

Background:

  • Embryonic stem (ES) cells possess pluripotency, differentiating into all fetal and adult lineages.
  • Genetic manipulation in murine ES cells is challenging due to time-consuming methods and inefficient delivery systems.
  • Current delivery methods are often limited to undifferentiated ES cell cultures.

Purpose of the Study:

  • To develop an efficient delivery system for biological materials into both undifferentiated and differentiating ES cells.
  • To overcome the limitations of existing genetic manipulation and delivery approaches in ES cells.
  • To establish a versatile tool for cellular labeling and cargo transfer in stem cells.

Main Methods:

  • Utilized a polystyrene microsphere-based system for cellular delivery.
  • Applied the system to undifferentiated and differentiating ES cells.
  • Delivered various cargos including fluorophores, proteins, and nucleic acids.

Main Results:

  • The microsphere system demonstrated efficient delivery of biological materials into ES cells.
  • Simultaneous cellular labeling and controlled cargo transfer were achieved.
  • No significant toxicity or loss of ES cell pluripotency was observed.
  • The system proved effective in various stem cell types, including trophoblast and neural stem cells.

Conclusions:

  • The developed polystyrene microsphere system offers an efficient and versatile solution for biological material delivery into ES cells.
  • This method overcomes key limitations of current techniques, supporting broader applications in stem cell research.
  • The system's effectiveness across different stem cell types highlights its potential impact on regenerative medicine and developmental biology.