Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Divalent cation binding to phospholipids: an EPR study.

J S Puskin

    The Journal of Membrane Biology
    |June 24, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Divalent cations like manganese (Mn2+) show high mobility when bound to acidic phospholipids in liposomes. Their binding affinity is influenced by monovalent ions and lipid composition, following Gouy-Chapman theory predictions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What can epidemiology tell us about risks at low doses?

    Radiation research·2007
    Same author

    The U.S. Environmental Protection Agency's assessment of risks from indoor radon.

    Health physics·2004
    Same author

    Smoking as a confounder in ecologic correlations of cancer mortality rates with average county radon levels.

    Health physics·2003
    Same author

    Radiation exposure and cancer: case study.

    American journal of epidemiology·2001
    Same author

    Adjustments to the baseline lung cancer mortality for radon-induced lung cancers in the BEIR VI risk models.

    Radiation research·2001
    Same author

    Risks from low doses of radiation.

    Science (New York, N.Y.)·1996
    Same journal

    In vitro Reconstitution of Syncytin-2 and MFSD2A Reveals their Functional Tethering in Membrane Fusion Initiation.

    The Journal of membrane biology·2026
    Same journal

    Structural Basis of SERCA Inhibition by Derivatives of di-tert-butylhydroquinone Revealed by X-ray Crystallography.

    The Journal of membrane biology·2026
    Same journal

    Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation.

    The Journal of membrane biology·2026
    Same journal

    Polyproline Modulates Membrane Translocation of Arginine-Rich Cell-Penetrating Peptides: Insights from Molecular Dynamics Simulations.

    The Journal of membrane biology·2026
    Same journal

    Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses.

    The Journal of membrane biology·2026
    Same journal

    Protonation of Key Acidic Residues Reveals Binding Features of PCABs to Gastric H, K-ATPase.

    The Journal of membrane biology·2026
    See all related articles

    Area of Science:

    • Biophysics
    • Physical Chemistry
    • Materials Science

    Background:

    • Liposomes are crucial in drug delivery and biomembrane research.
    • Understanding cation-lipid interactions is key to controlling liposome stability and function.

    Purpose of the Study:

    • To investigate divalent cation binding to phospholipid liposomes.
    • To determine the influence of monovalent ions and lipid composition on cation-liposome interactions.

    Main Methods:

    • Electron paramagnetic spectroscopy (EPR) was used to study manganese (Mn2+) association with liposomes.
    • Apparent affinities (KA) were measured as a function of monovalent electrolyte concentration.

    Main Results:

    • Divalent cations bound to acidic phospholipids exhibit high mobility.

    Related Experiment Videos

  • Monovalent cations with smaller hydrated radii more effectively displaced Mn2+.
  • Mn2+ affinity followed Gouy-Chapman theory predictions for cardiolipin, but deviated for phosphatidylserine and phosphatidic acid.
  • Mn-binding strength in mixed vesicles correlated with charged phospholipid content.

    • Conclusions:

    • Lipid composition and ionic environment significantly modulate divalent cation binding to liposomes.
    • A 1:2 divalent cation-phospholipid interaction model is supported.
    • Findings have implications for liposome design and stability in biological systems.