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Related Experiment Video

Updated: Jun 21, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Amplified electrochemical aptasensor for thrombin based on bio-barcode method.

Xiaoru Zhang1, Baoping Qi, Ying Li

  • 1Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Biosensors & Bioelectronics
|July 18, 2009
PubMed
Summary
This summary is machine-generated.

An electrochemical aptasensor was developed for sensitive thrombin detection using a bio-barcode amplification assay. This novel sensor achieves a low detection limit for thrombin, showing high selectivity against other proteins.

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Last Updated: Jun 21, 2026

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

  • Biomedical Engineering
  • Electrochemistry
  • Nanotechnology

Background:

  • Thrombin is a key biomarker in hemostasis and thrombosis.
  • Sensitive and selective detection of thrombin is crucial for diagnosing and monitoring coagulation disorders.
  • Existing detection methods often lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a highly sensitive electrochemical aptasensor for thrombin detection.
  • To utilize a bio-barcode amplification assay for enhanced signal generation.
  • To establish a robust platform for clinical diagnostics.

Main Methods:

  • Immobilization of capture DNA aptamer on a gold electrode.
  • Fabrication of a sandwich complex involving thrombin and DNA-functionalized gold nanoparticles.
  • Hybridization with lead-SNP-labeled DNA and detection via differential pulse anodic stripping voltammetry (DPASV).

Main Results:

  • Achieved a highly sensitive detection limit of 6.2x10⁻¹⁵ M for thrombin.
  • Demonstrated excellent selectivity of the aptasensor against other proteins.
  • The developed aptasensor showed reliable performance under optimized conditions.

Conclusions:

  • The developed electrochemical aptasensor offers a sensitive and selective method for thrombin detection.
  • The bio-barcode amplification strategy significantly enhances detection capabilities.
  • This platform holds potential for developing advanced diagnostic tools for coagulation-related diseases.