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Updated: Jun 21, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
Published on: September 6, 2019
Enzyme synthesis of oligosaccharides using cashew apple juice as substrate
Maria Cristiane Rabelo1, Claudia P M L Fontes, Sueli Rodrigues
1Departamento de Tecnologia de Alimentos (UFC), Campus do Pici, Bloco 858, Caixa Postal 12168, CEP 60021-970, Fortaleza-CE, Brazil.
Abstract:
The use of agriculture substrates in industrial biotechnological processes has been increasing because of their low cost. In this work, the use of clarified cashew apple juice was investigated as substrate for enzyme synthesis of prebiotic oligosaccharide. The results showed that cashew apple juice is a good source of reducing sugars and can be used as substrate for the production of dextransucrase by Leuconostoc citreum B-742 for the synthesis of oligosaccharides using the crude enzyme. Optimal oligosaccharide yield (approximately 80%) was obtained for sucrose concentrations lower than 60 g/L and reducing sugar concentrations higher than 100 g/L.
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