Related Experiment Video
Updated: Jun 21, 2026

A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
An amperometric D-amino acid biosensor prepared with a thermostable D-proline dehydrogenase and a carbon
Yuji Tani1, Yukiko Itoyama, Kenichi Nishi
1Department of Chemical Science and Technology, Institute of Science and Tecnhology, The University of Tokushima, Japan.
Abstract:
Carbon nanotube (CNT) gel, which is composed of a mixture of single-wall CNT, an ionic liquid, and a thermostable D-proline dehydrogenase (D-Pro DH) immobilized electrode was utilized for the determination of D-amino acids (DAAs) in food samples. When a critical comparison with CNT, Ketjen Black (KB), and carbon powder (CP) was also carried out, the CNT/D-Pro DH immobilized electrode showed the highest sensitivity and the lowest detection limit of D-proline. In addition, the CNT/D-Pro DH immobilized electrode was applied to detection of DAAs in rice wine and vinegar samples. The concentrations of DAAs in rice wine and vinegar samples were 0.0210 +/- 0.0001 and 0.55 +/- 0.05 mmol L(-1), respectively.

