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Correlation between DNA quantities in agarose gel and chromatographic areas of photographed DNA bands
M Chinami1, E Tanikawa, H Hachisuka
1Department of Virology, Kurume University School of Medicine, Japan.
The Kurume Medical Journal
|January 1, 1991
Abstract:
To quantitate DNA from photographs, correlations between chromato-scanned (in the fluorescence mode) and virtual areas from white DNA bands on photographs were examined. A linear correlation between the two measurements with coefficients higher than 0.89 was observed. The dynamic range of the fluorescence detection yielded good linearity for a wide range of amounts of DNA (microgram-ng) on the photograph.