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Low concentrations of interferon-gamma stimulate DNA synthesis in normal human keratinocytes during the initial
K Okubo1, T Karashima, H Hachisuka
1Department of Dermatology, Kurume University School of Medicine, Japan.
Abstract:
High concentrations of interferons can inhibit keratinocyte proliferation and induce the expression of HLA-DR antigen on keratinocytes. In the present study, the effects of low concentrations of recombinant human interferon alpha, beta and gamma were examined on DNA synthesis and the expression of HLA-DR and bullous pemphigoid (BP) antigens in normal human keratinocytes. Low concentrations of interferons induced DNA synthesis in normal human keratinocytes when epidermal cell growth factor and low Ca++ were used during the initial stages of culturing. This result indicates that physiological concentrations of interferons can induce keratinocyte activation.