Related Experiment Video
Updated: Jun 21, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
A gas diffusion technique coupled with flow injection systems. Optimization of the process in its application to the
María-Jesús Almendral-Parra1, Angel Alonso-Mateos, María-Socorro Fuentes-Prieto
1Departamento de Química Analítica, Nutrición y Bromatología, University of Salamanca, Plaza de la Merced s/n, Salamanca, 37008, Spain. almendral@usal.es
Abstract:
A novel flow injection-gas-diffusion (GD-FI) system has been developed for the on-line analysis of ammonium ion in waters with fluorimetric detection, using an acceptor solution containing the Eosin-Bluish (EB) acid-base indicator. This, together with optimization of the process of gas transfer through the membrane, increases the sensitivity of the method to a considerable extent. Under optimum conditions, it is possible to determine the analyte within the 0.02-1.5 mg l(-1) range, with a limit of detection of 5 microg l(-1) and relative standard deviations (n = 12, [NH (4) (+) ] = 50 microg l(-1) and 0.05 microg l(-1)) of 3.4% and 3.0% respectively. The determination rate was 15 samples per hour.
More Related Videos
Related Concept Videos
Gas Chromatography: Sample Injection Systems
Two primary injection methods are used...
Atomic Fluorescence Spectroscopy
Gas Chromatography: Types of Detectors-II
Atomic Absorption Spectroscopy: Atomization Methods
Flame Photometry: Lab

