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Generation of Human Chimeric Antigen Receptor Regulatory T Cells
Published on: January 3, 2025
Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.
Zhe Shi1, Yusuke Okuno, Muhaimin Rifa'i
1Department of Immunology, Nagoya University Graduate School of Medicine, Tsurumai-cho, Showa-ku, Nagoya, Japan.
European Journal of Immunology
|July 18, 2009
Summary
Human CD8(+)CXCR3(+) T cells are identified as the functional equivalent of mouse CD8(+)CD122(+) regulatory T cells (Treg). These cells play a crucial role in immune regulation and homeostasis.
Area of Science:
- Immunology
- T cell biology
Background:
- Murine CD8(+)CD122(+) Treg are vital for immune homeostasis.
- Human CD8(+) Treg counterparts to murine CD8(+)CD122(+) Treg remain unidentified due to differing CD8 and CD122 expression patterns.
Purpose of the Study:
- To identify the human equivalent of murine CD8(+)CD122(+) regulatory T cells (Treg).
- To characterize the function and phenotype of potential human Treg counterparts.
Main Methods:
- DNA microarray analysis to compare gene expression profiles of murine CD8(+)CD122(+) and CD8(+)CD122(-) cells.
- In vivo and in vitro assays to evaluate regulatory activities of identified cell populations.
- Analysis of CD122 and CXCR3 expression on murine and human CD8(+) T cells.
Main Results:
- CXCR3 is preferentially expressed in murine CD8(+)CD122(+) cells.
- A distinct population of CD8(+)CXCR3(+) cells in mice exhibits regulatory functions similar to CD8(+)CD122(+) Treg.
- Human CD8(+) T cells express CXCR3 but not CD122, and CD8(+)CXCR3(+) cells demonstrate regulatory activity by producing IL-10 and suppressing IFN-gamma.
Conclusions:
- Human CD8(+)CXCR3(+) T cells are identified as the functional counterparts of murine CD8(+)CD122(+) Treg.
- This finding clarifies the identification and function of a key regulatory T cell subset in humans.
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