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Related Experiment Videos

Polypeptide synthesis in simian virus 5-infected cells.

R W Peluso, R A Lamb, P W Choppin

    Journal of Virology
    |July 1, 1977
    PubMed
    Summary

    Simian virus 5 infection alters polypeptide synthesis, with viral proteins produced in proportions found in virions. Some host cell polypeptides may be enhanced during infection, potentially aiding virus replication.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Simian virus 5 (SV5) is a paramyxovirus that infects various cell types.
    • Understanding viral protein synthesis is crucial for comprehending viral replication and pathogenesis.

    Purpose of the Study:

    • To identify and characterize polypeptide synthesis in SV5-infected cells.
    • To investigate the regulation of viral and host cell protein production during infection.

    Main Methods:

    • High-resolution polyacrylamide slab gel electrophoresis was used to analyze polypeptide synthesis.
    • Pulse-chase experiments were employed to study protein precursor processing.

    Main Results:

    • All known SV5 viral polypeptides were identified in infected cells.
    • Viral polypeptides were synthesized in proportions similar to those in virions, suggesting controlled synthesis.
    • A precursor glycoprotein (F(0)) was identified and shown to be cleaved into F(1) and F(2).
    • Five non-structural polypeptides (I-V) were observed, with one (V) potentially being a viral nonstructural protein.
    • Polypeptides I-IV appeared to be host cell proteins whose synthesis might be enhanced by infection.

    Conclusions:

    • SV5 infection involves controlled synthesis of viral polypeptides.
    • Host cell protein synthesis can be modulated during SV5 infection, with some host proteins potentially playing a role in virus replication.

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